Se a streamer ainda não tiver nenhum assinante, ele só poderá carregar um emoticon como afiliado. Para cada outro, você já precisa de pontos de assinantes. Você receberá pontos de assinante com assinaturas.
Here you can find all other guidelines and details about Twitch Subscriber Emoticons:
On your Twitch Dashboard you will find the menu item “Emoticons” in “Settings”. If you click on “Edit” you can upload your Twitch Emotes as PNG. In the same menu you will also find options to change or delete already existing emoticons. When uploading the emoticon you can assign a code with up to 20 digits to activate the Twitch Emote. This code may contain the characters A-Z and the digits 0-9.
Twitch emotes must be uploaded as PNG and should have a transparent background. They are available in three different sizes, for each of which a single PNG file must be uploaded: 28 x 28, 56 x 56 and 112 x 112. The individual files shouldn’t be larger than 25 kb. A detailed guide with more information can be found here on Twitch.